A new research direction, started in the last century
in vegetal and animal biology is that of cultivating cells, tissues
or protoplasts on artificial media. This technique became an important
research domain in cultivating various living organisms.
Cell culture is the process by which prokaryotic or
eukaryotic cells are grown under controlled conditions. In practice
the term "cell culture" refers to the culturing of cells derived
from multicellular eukaryotes.
Tissue culture is the growth of tissues and/or cells
separate from the organism. This is typically facilitated via use of
a liquid, semi-solid, or solid growth media. The more specific term
plant tissue culture is used for plants.
Organ culture is a development from tissue culture
methods of research; in this case, the organ culture is able to accurately
model functions of an organ in various states and conditions by the
use of the actual in vitro organ itself.
Parts of an organ or a whole organ can be cultured
in artificial conditions, the so called in vitro techniques. The main
objective is to maintain the structure of the tissue and direct it towards
normal development. It is very important that the tissue is not damaged.
This technique supposes careful handling of the vegetal material and
of all the instruments used.
In our Micropropagation and Germoplasm Preservation
Laboratory of “Anastasie Fatu” Botanical Garden, some rare
and damaged plant species will be multiplicated, as well as some varieties
of the plants existing in our collections on one hand and a seed collection
preserved on short and long term, on the other hand.
Micropropagation and Germoplasm Preservation Laboratory
is tooled with: aspirant fume hood, cabinet with horizontal laminar
flow, bactericide lamp, KBW 400 plant growth chamber, Velp hot plate
magnetic stirrer, Raypa magnetic stirrer for small volumes, Adam analytical
balance, vessel washing machine, AES 75 autoclave, laboratory refractometer,
stereomicroscopes with digital photo camera, Labconco clear Drying Chamber